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KMID : 0603820090150020113
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Journal of Experimental & Biomedical Science
2009 Volume.15 No. 2 p.113 ~ p.118
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2-Deoxy-D-glucose Regulates Dedifferentiation but not Cyclooxygenase-2 Expression through Reorganization of Actin Cytoskeletal Architecture in Rabbit Articular Chondrocytes
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Yu Seon-Mi
Kim Song-Ja
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Abstract
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Actin cytoskeletal architecture is believed to be a crucially important modulator of chondrocyte phenotype. 2DG (2-Dexoy-D-glucose) induces reorganization of actin cytoskeletal architecture in chondrocytes. In this study, we have investigated the effects of 2DG on dedifferentiation and inflammation via reorganization of cytoskeletal architecture in rabbit articular chondrocytes, with a focus on p38 kinase pathway. Treatment of 2DG alone reduced type Ⅱ collagen and COX-2 expression in chondrocytes. But, 2DG reduced type Ⅱ collagen was recovered by CD, disruptor of actin cytoskeletal architecture, whereas did not affect on COX-2 expression and production of PGE2 compared with 2DG alone treated cells. Treatment of 2DG with JAS, inducer of cytoskeletal architecture polymerization, accelerated reduction of type Ⅱ collagen expression and synthesis of proteoglycan but did not affect on COX-2 expression and production of PGE2. Also, 2DG stimulated activation of p38 kinase. This result showed that 2DG regulates type Ⅱ collagen but not cyclooxygenase-2 expression through reorganization of cytoskeletal architecture via p38 kinase pathway in rabbit articular chondrocytes.
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KEYWORD
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Actin, Cytoskeleton, Dedifferentiation
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